Gut Brain Barrier

Gut-Brain Barrier | Strain Specific Research

Probiotic strains have very specific functions and should be chosen accordingly. Additionally, some strains should not be put together in capsules or powder due to their incompatibility. It is a complex science, and it is important to trust the source and their knowledge.

Our partners, Winclove, are a family-owned company established in Amsterdam. They specialise in the research, development and manufacturing of probiotic formulations. Below, we highlight their research into gut-brain barrier probiotic strains.

In Vitro Breakdown of Lipopolysaccharides (LPS)

Bacteria have the ability to break down LPS with the enzyme alkaline phosphatase. Several bacterial strains have been shown to produce alkaline phosphatase in vitro, thus indicating differing capacities to breakdown LPS.

LPS breakdown

In Vitro Increased Trans Epithelial Electric Resistance (TEER)

After an inflammatory stressor, the TEER can be seen to decrease, indicating a reduction in gut barrier integrity (see 'with stressor control'). Bacterial strains exhibit differing capacities for modulating this response, as seen in the graph below, compared to controls.

Intestinal Barrier

In Vitro Reduction of Mast Cell Degranulation

Several strains are able to significantly reduce the secretion of β-hexosaminidase, which is an indicator of mast cell degranulation. Mast cells release proteases, which rearrange tight junctions, histamine, and inflammatory cytokines, all of which increase epithelial permeability.

Mast Cell Degranulation

In Vitro Strengthening of the Immune System

Several strains have been shown to stimulate production of the anti-inflammatory cytokine IL-10 in vitro. The graph below shows that certain strains stimulate IL-10 production to levels 200 to 300% of the stimulated control.


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